Introduction to customProDB
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چکیده
3 Building database from a single sample 4 3.1 Filtering based on transcript expression . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.2 Variation annotation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 3.2.1 SNVs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3.2.2 INDELs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 3.3 Splice junction analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
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customProDB: an R package to generate customized protein databases from RNA-Seq data for proteomics search
UNLABELLED Database search is the most widely used approach for peptide and protein identification in mass spectrometry-based proteomics studies. Our previous study showed that sample-specific protein databases derived from RNA-Seq data can better approximate the real protein pools in the samples and thus improve protein identification. More importantly, single nucleotide variations, short inse...
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تاریخ انتشار 2015